An effective placental cotyledons proteins extraction method for 2D gel electrophoresis

2D Gel Electrophoresis - An Overview

Gel Electrophoresis of Proteins

Gel electrophoresis is a widely known group of techniques used to separate and identify macromolecules as DNA, RNA, or proteins based on size, form, or isoelectric point. The separation of molecules by electrophoresis is based on the fact that charged molecules migrate through a gel matrix upon application of an electric field. These techniques have become a main tool in biochemistry, molecular...

Plenary: an Effective Skeletal Muscle Pre-Fractionation Method to Remove Abundant Structural Proteins for Optimized Two Dimensional Gel Electrophoresis

Proteomic analysis of biological samples in disease models or therapeutic intervention studies requires the ability to detect and identify biologically relevant proteins present in relatively low concentrations. The detection and analysis of these low-level proteins is hindered by the presence of a few proteins that are expressed in relatively high concentrations. In the case of muscle tissue, ...

Behavior of the Serum proteins in starch-agar-gel electrophoresis

Segmentation of 2d-gel Electrophoresis Images

Introduction Two-Dimensional Gel Electrophoresis technique is a convenient and well-established method to separate thousands of proteins on polyacrylamide gels, according to the differences in their net charge and their molecular mass [1]. Its digital output is an image which depicts proteins as bright or dark spots over a noisy and inhomogeneous background. Each protein is characterized by its...